In This Issue: Volume 15, Issue 1
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Cultured Madin-Darby canine kidney (MDCK) epithelial cells labeled with propidium iodide and Alexa Fluor 50 conjugated to phalloidin, which target DNA and filamentous actin, respectively. In addition, the cells were first transfected with a pEYFP-mitochondria plasmid subcellular localization vector, thus localizing a yellow fluorescent protein tag to the intracellular mitochondrial network. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles. – Courtesy of Michael W. Davidson, National High Magnetic Field Laboratory © 2012, The Florida State University, Tallahassee, www.microscopy.fsu.edu
BioProcessing Journal (ISSN 1538-8786) is a peer-reviewed, quarterly publication that features the latest technological advancements and best practices for the development and production of safe and effective biologics.