In This Issue: Volume 13, Issue 2
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Mink uterus endometrium epithelial cells (GMMe) were immunofluorescently labeled with primary anti-vimentin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Marina Blue. Alexa Fluor 594 conjugated to phalloidin (cytoskeletal F-actin network) and SYTOX Green (nuclei) were also used to stain this epithelial cell culture. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles. — Image provided by Michael W. Davidson, National High Magnetic Field Laboratory, (www.microscopy.fsu.edu), The Florida State University, Tallahassee.
BioProcessing Journal (ISSN 1538-8786) is a peer-reviewed, quarterly publication that features the latest technological advancements and best practices for the development and production of safe and effective biologics.