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Cover image:
Nuclear histone proteins were targeted in Madin-Darby ovine kidney (MDOK) epithelial cells with mouse anti-histone (pan) mAbs, which were imaged with goat anti-mouse FAbs conjugated to Texas Red. The specimen was simultaneously labeled for the Golgi complex with Alexa Fluor 488 conjugated to goat secondary antibodies that target rabbit anti-giantin, and Alexa Fluor 350, conjugated to phalloidin, was utilized to counterstain the cytoskeletal F-actin network. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.


Image provided by Michael W. Davidson, National High Magnetic Field Laboratory,
The Florida State University, Tallahassee, Florida USA

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