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Cover image:
This microtubule network was visualized in the culture of African green monkey kidney fibroblast cells (COS-7) that were transformed with SV40. Immunofluorescent labeling was done with primary anti-tubulin mouse MAbs followed by goat anti-mouse FAbs conjugated to Cy3. In addition, F-actin and the cell nuclei were targeted with Alexa Fluor 488 conjugated to phalloidin and DAPI, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.


Image provided by Michael W. Davidson, National High Magnetic Field Laboratory,
The Florida State University, Tallahassee, Florida USA

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