Protein A Affinity Captures of Monoclonal Antibodies from Cell Culture Supernatants on 96-Column Microfilter Plates for Medium Throughput Analytical Support of Bioprocess Development

by Jacob Bongers, PhD, Paul N. Boyd, Ruth De Vries, Jim D. Faulkner, Marcia M. Federici, Joselina Gorniak, Anna E. Hills, Maggie M. Huang, Cindee Levow, Gregory J. Mazzola, Allison W. Moore, La Donna Nugent, Leonard T. Olszewski, Ashvin K. Patel, Kankindi Rwego, Thomas M. Smith, Mark Strohsacker, and Eileen Wilson
Volume 2, Issue 2 (March/April 2003)

Large scale genomics spurred the development of massively parallel methods of automated DNA purification and sequencing. These methods started with the 1962 development of a 96-well microtiter plate for miniature-scale serology studies. This simple laboratory device has since been greatly modified and extended to include numerous specialty multiwell plates contructed and/or coated with different materials for various purposes. The original 96-well (8x12 matrix) has expanded to include 384-well and higher densities. More recently, functionality and versatility have been greatly augmented by the incorporation of a filter, or thin membrane, into the bottom of the well. These multiwell microfilter plates can thus be employed in a flow-through mode, in addition to the familiar "put in" and "take out" pipetting and rinsing steps associated with traditional enzyme-linked immunosorbent assay (ELISA) microtiter plate methods...

Citation:
Bongers J et al. Protein A Affinity Captures of Monoclonal Antibodies from Cell Culture Supernatants on 96-Column Microfilter Plates for Medium Throughput Analytical Support of Bioprocess Development. BioProcess J, 2003; 2(2): 41-47. http://dx.doi.org/10.12665/J22.Bongers.