Replacement of Wheat Peptone During Development of a Defined Medium for Recombinant Sp2/0 Cells

by Michael C. Borys, PhD, Katherine Hughes, and Jon M. Ryan, PhD
Volume 2, Issue 2 (March/April 2003)

Peptones are protein digests composed mainly of amino acids and small peptides. Peptones have been used in mammalian cell culture medium as a serum substitute to enhance cell growth and product formation. The first part of this study describes our evaluation of peptones from different sources (soy, wheat, yeast, and casein) on the cell growth and productivity of Sp2/0 myeloma cells expressing recombinant prourokinase (r-ProUK). The results of these studies demonstrated that wheat peptone was the most effective plant peptone to increase r-ProUK yield. Addition of 2 g/L wheat peptone to the culture medium increased batch r-ProUK production between 28-67% compared to cells grown in the absence of peptone supplements. Peptones did not increase cell productivity, but increases r-ProUK yield through increased culture longevity...

Citation:
Borys MC, Hughes K, Ryan JM. Replacement of Wheat Peptone During Development of a Defined Medium for Recombinant Sp2/0 Cells. BioProcess J, 2003; 2(2): 57-64. http://dx.doi.org/10.12665/J22.Borys.