Electron Microscopy: Current Techniques Used in Product Safety

by Euan W. Milne
Volume 2, Issue 2 (March/April 2003)

Electron microscopy (EM) provides data for viral clearance studies, information on the presence and quantitation of endogenous retroviruses, and the detection and characterization of other potential contaminants. The technique is favored in this field because it is simple, reliable, and can give reliable quantitation for risk assessments. This article describes the main EM techniques currently used for testing cell cultures, culture supernatants, and bulk harvests. It also includes an in-depth description of a thin sectioning technique used to estimate virus titre in culture supernatants and bulk harvests...

Citation:
Milne EW. Electron Microscopy: Current Techniques Used in Product Safety. BioProcess J, 2003; 2(2): 65-68. http://dx.doi.org/10.12665/J22.Milne.