Quantitative Analysis of Monoclonal Antibodies for Nonglycosylated Heavy Chains with a Microfluidic Chip

by Joselina G. Gorniak, Jacob Bongers, PhD, Mark Strohsacker, and Leonard T. Olszewski, PhD
Volume 2, Issue 2 (March/April 2003)

Glycosylation, a posttranslational modification that adds sugars to proteins, is required by many proteins to function properly. Glycosylation can modulate the biological activities of monoclonal antibodies (MAbs), including certain effector functions in the Fc region of IgG antibodies. Monoclonal antibodies produced at higher expression levels by mammalian cell culture may contain small amounts of nonglycosylated heavy chain (NGHC). Recent cell culture mini-reactor studies have shed light on the process parameters that most affect the occurrence of NGHC, and have greatly minimized NGHC levels in IgG MAb products...

Citation:
Gorniak JG, Bongers J, Strohsacker M, Olszewski LT. Quantitative Analysis of Monoclonal Antibodies for Nonglycosylated Heavy Chains with a Microfluidic Chip. BioProcess J, 2003; 2(2): 78-82. http://dx.doi.org/10.12665/J22.Gorniak.