Transfected Cell Line Enrichment Using the Gel Microdrop (GMD) Secretion Assay

by Yevgenya Akselband, PhD, Jan Trnovsky, PhD, and Patricia McGrath
Volume 2, Issue 2 (March/April 2003)

The Gel Microdrop (GMD) Secretion Assay involves encapsulating cells within a biotinylated agarose matrix, followed by capture and detection of cell-secreted molecules with fluorescent markers. This technology differs from other encapsulation methods in that the small size of the microdrop (<50 μm diameter) creates a defined microenvironment around the cell without impeding the fusion of nutrients, antibodies, or nucleic acid probes into the GMDs, or the diffusion of secreted products out of the GMDs. Large numbers of GMDs can be readily analyzed using flow cytometry, and sub-populations of rare or high-secreting cells, as small as 0.1%, can be detected and recovered in one day. This assay format is a rapid alternative to limited dilution cloning (LDC)...

Citation:
Akselband Y, Trnovsky J, McGrath P. Transfected Cell Line Enrichment Using the Gel Microdrop (GMD) Secretion Assay. BioProcess J, 2003; 2(2): 83-88. http://dx.doi.org/10.12665/J22.Akselband.