Advances in High Cell Density Culture Technology Using the Sf-9 Insect Cell/Baculovirus Expression System — The Fed-Batch Approach

by Cynthia B. Elias, PhD, Arno Zeiser, and Amine Kamen, PhD
Volume 2, Issue 1 (January/February 2003)

The Sf-9 insect cell/baculovirus expression system is one of the most commonly used protein expression systems. It is the preferred system for generating large amounts of protein in a short period of time, and it has been successfully used to express several hundreds of different proteins. A representative list of the different proteins made in our laboratory over the past decade with the Sf-9 insect cell/BEVS system is given in Table 1. These proteins are often used in drug screening studies and structure function analysis. Proteins intended for therapeutic purposes are not normally produced using this technology, although a few examples do exist. There is also an unexplored potential for the cells to be used for the production of recombinant viral vectors. Recent reports demonstrating the ability of baculoviruses to express proteins in mammalian cells, with mammalian promoters, indicate that BEVS technology might soon have a major role to play in the field of gene delivery...

Citation:
Elias CB, Zeiser A, Kamen A. Advances in High Cell Density Culture Technology Using the Sf-9 Insect Cell/Baculovirus Expression System — The Fed-Batch Approach. BioProcess J, 2003; 2(1): 22-29.