Adenovirus Type 5 (Ad5) Chromatographic Purification Process at the 20 L Scale

by Normand Arcand, Alice Bernier, Julia Transfiguracion, Danielle Jacob, Helene Coelho, and Amine Kamen, PhD
Volume 2, Issue 1 (January/February 2003)

Recombinant adenovirus are attractive as vectors for gene therapy because: they exhibit wide tissue tropism and high transduction efficiency; adenovirus cultures can reach high specific titers (10^10 VP/mL), and; their use in the treatment of cancer and other serious diseases is valuable. A primary mode of adenovirus purification continues to be CsCl density gradient centrifugation...

Citation:
Arcand N, Bernier A, Transfiguracion J, Jacob D, Coelho H, Kamen A. Adenovirus Type 5 (Ad5) Chromatographic Purification Process at the 20 L Scale. BioProcess J, 2003; 2(1): 72-75.