A Short-Term Field Use and Shipping Stability Study of a Wild Type Ad5 Adenoviral Reference Material

by Kodjo Adadevoh, Maria Croyle, Daniel Malarme, Edwige Bonfils, and Mark A. Bowe, PhD
Volume 1, Issue 3 (Fall 2002)

Adenoviral vectors for gene delivery are being tested in the clinic for a number of indications and therapeutic uses. In order to facilitate the comparison of studies from different laboratories, the Adenovirus Reference Material Working Group (ARMWG) has developed a reference testing reagent, which will be referred to as the Wild Type Ad5 Adenoviral Reference Material (ARM). This ARM will allow laboratories to standardize in-house controls employed in assays for the determination of particle concentration and infectious titer of their own adenoviral preparations. As part of this project, short-term field use and shipping studies were performed on the ARM. The virus was found to be stable under simulated shipping conditions, for one thaw after shipping, and at 4 °C for up to four hours after thawing. However, there was evidence of aggregation in some vials with repeated freeze-thaw cycles. Therefore, we recommend that each vial be treated as a single-use aliquot, and that it be used within four hours of thawing...

Citation:
Adadevoh K, Croyle M, Malarme D, Bonfils E, Bowe MA. A Short-Term Field Use and Shipping Stability Study of a Wild Type Ad5 Adenoviral Reference Material. BioProcess J, 2002; 1(3): 62-69.