A Rapid Method for the Capture and Purification of a Protease Sensitive Protein

by Christopher W. Kemp, PhD
Volume 1, Issue 2 (Summer 2002)

The baculovirus expression vector system (BEVS) is one method utilized for the production of recombinant proteins, and typically works without significant difficulties. However, some proteins are produced in insoluble forms, and degradation can occur. This article will focus on this degradation issue, and present a method to stabilize a protease-sensitive protein that has been produced at the 40-liter scale...

Citation:
Kemp CW. A Rapid Method for the Capture and Purification of a Protease Sensitive Protein. BioProcess J, 2002; 1(2): 36-39.