Large-Scale Production of Active Serine-Threonine Kinases of the MAPK Pathway Using the Baculovirus System

by Steven L. Nguyen and Wai-Ping Fung-Leung, PhD
Volume 1, Issue 2 (Summer 2002)

Serine-threonine kinases of the Mitogen Activated Protein Kinase (MAPK) pathway represent potential drug targets for a wide range of diseases. As part of an effort to understand the biology of the pathways, several human serine-threonine MAPKs were produced. Optimization and modification of current methodologies used in the baculovirus expression system resulted in the generation of large amounts of active MAPKs. Compounds found to inhibit the MAPKs in vitro, subsequently showed activity in cell-based assays and animal models. The processes to be discussed were developed to yield large quantities of three active human serine-threonine MAPKs by the baculovirus expression system...

Citation:
Nguyen SL, Fung-Leung W-P. Large-Scale Production of Active Serine-Threonine Kinases of the MAPK Pathway Using the Baculovirus System. BioProcess J, 2002; 1(2): 43-46.