Bovine Serum Albumin Partitioning in Aqueous Two-Phase Systems: Effects of Variables and Optimization

by Nangarthody Sindhu, Sivakumar Kalaivani, and Iyyaswami Regupathi
Volume 12, Issue 1 (Spring 2013)

The objective of this study was to optimize process conditions for the effective partitioning of bovine serum albumin (BSA) using response surface methodology (RSM). Initially, four different salts (tripotassium citrate, tripotassium phosphate, sodium carbonate, and sodium sulphate) were tested for the ability to partition BSA. Among the salts chosen, tripotassium citrate was observed to yield a high partition coefficient. The effect of phase forming components: concentration, PEG molecular weight, and pH were studied for a PEG/tripotassium citrate system and the information obtained was utilized to fix the ranges in RSM studies. Four different independent variables (PEG 2000, tripotassium citrate, NaCl concentrations, and pH) were considered for RSM studies and the responses generated were partition coefficient (k) and percentage yield. A statistical model was developed and the values obtained were 99 % within the confidence level. Optimal conditions of the system were found as: 0.25 M sodium chloride, 32 % PEG 2000 (w/w), 16 % tripotassium citrate (w/w), pH 6.0, a partition coefficient of 6.03, a recovery of 91.76 %, and a controlled operating temperature of 303.15 K...

Citation:
Sindhu N, Kalaivani S, Regupathi I. Bovine Serum Albumin Partitioning in Aqueous Two-Phase Systems: Effects of Variables and Optimization. BioProcess J, 2013; 12(1): 29-41. dx.doi.org/10.12665/J121.Regupathi.

Posted online May 30, 2013.