Effective Site-Specific Isotopic Labeling: Expression Optimization Using Specialized Media

by Kenneth C. Bonanno
Volume 12, Issue 1 (Spring 2013)

For decades, recombinant genes have been expressed in a variety of cellular systems to generate protein reagents that are the potential targets for new small-molecule drugs. As these targets become more complex, researchers have developed innovative methods to study the structure of these proteins and their interactions with potential drugs. BioExpress® 2000 media (Cambridge Isotope Laboratories, CIL) incorporates isotope-labeled amino acids into recombinant proteins expressed in baculovirus-infected insect cells to assist nuclear magnetic resonance (NMR) structural studies. In order to use this labeled media most efficiently, expression conditions must be optimized at a small-scale prior to production...

Citation:
Bonanno KC. Effective Site-Specific Isotopic Labeling: Expression Optimization Using Specialized Media. BioProcess J, 2013; 12(1): 43-46. http://dx.doi.org/10.12665/J121.Bonanno.

Posted online May 30, 2013.