Design and Optimization of a Purification Process for MY32/Ls Protein Solubilizing Inclusion Bodies for a New Vaccine Against Sea Lice

by Carlos Perez Heredia, Nemecio González Fernández, Eladio Salazar Gómez, Eulogio Pimentel Vázquez, Yamila Carpio González, and Miladys Limonta Fernández
Volume 14, Issue 1 (Spring 2015)

Sea lice are the most problematic marine pathogens the salmon industry has to deal with, significantly affecting Europe and America. The worst offenders are genera: Pseudocaligus, Caligus, and Lepeophtheirus. Over €305 million in losses are estimated. Recent results have suggested that subolesin/akirin/myosin32 are good candidate antigens for the control of arthropod infestations such as sea lice. The aim of this study was to design and optimize the purification step of MY32/Ls protein to obtain the active pharmaceutical ingredient against sea lice. Non-chromatographic purification strategies were employed, based on published works, to establish rupture, washing, solubilization, and refolding conditions...

Citation:
Heredia CP, Fernández NG, Gómez ES, Vázquez EP, González YC, Fernández ML. Design and optimization of a purification process for MY32/Ls protein solubilizing inclusion bodies for a new vaccine against sea lice. BioProcess J, 2015; 14(1): 49–59. http://dx.doi.org/10.12665/J141.Heredia.

Posted online May 1, 2015.