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Opinion
POP Talk: Prepare, Organize, Present

by Tom Kost, PhD
Volume 15, Issue 4 (Winter 2016/2017)

You have worked tirelessly on your project and an opportunity arises to present your results. It might be a group meeting, departmental seminar, job interview, or scientific conference. Alternatively, you may need to raise money to fund your research, start a biotechnology company, or market a new product. In each of these instances, delivering your message effectively is essential to achieving your goal and earning the respect and admiration of colleagues...

Citation:
Kost T. POP talk: prepare, organize, present. BioProcess J, 2017; 15(4): 4–5. https://doi.org/10.12665/J154.Kost.

Posted online February 15, 2017.

 
Opinion

by Donald L. Jarvis, PhD
Volume 15, Issue 3 (Fall 2016)

I have to begin with a disclosure. I am a card-carrying, old-school, hypercritical academic who has always been among the first to complain about going to a conference and having to sit through an infomercial disguised as a science talk. But, perhaps I have a disguise of my own. I actually enjoy hearing talks focused on applied research and product development at least as much as more “academic” talks. I admire and respect the practical application of brilliant science. I believe that’s what scientists are supposed to do...

Citation:
Jarvis DL. Opinion. BioProcess J, 2016; 15(3): 5. https://doi.org/10.12665/J153.Jarvis.

Posted online November 15, 2016.

 
Low-Pressure Liquid Chromatography Using the EcoPrime® With Enhanced Buffer In-Line Dilution

by Mary Jo Wojtusik, PhD and Kurt Willner
Volume 15, Issue 3 (Fall 2016)

Chromatography and buffer in-line dilution (BID) are integrated on the same low-pressure liquid chromatography (LPLC) system combining two unit operations into a single, space-saving platform. The EcoPrime’s BID option will significantly reduce buffer storage and lower operating expenses associated with buffer preparation...

Citation:
Wojtusik MJ, Willner K. Low-pressure liquid chromatography using the EcoPrime® with enhanced buffer in-line dilution. BioProcess J, 2016; 15(3): 14–9. https://doi.org/10.12665/J153.Wojtusik.

Posted online November 15, 2016.

 
Quality Risk Management (QRM): Part I – Identifying, Evaluating, and Mitigating Threat Risks to Biopharmaceutical Enterprises

by Mark F. Witcher
Volume 15, Issue 3 (Fall 2016)

The FDA’s ICH Q9 quality risk management (QRM) guidance material is the foundation for understanding and evaluating patient risks associated with developing and manufacturing pharmaceuticals. This three-part paper describes approaches a team of subject matter experts (SMEs) can use for implementing two important applications of QRM. Part I provides a method for identifying and remediating threat risks that may affect the product’s quality or other important aspects of a manufacturing enterprise’s lifecycle, from product research and development to commercial manufacturing. The second QRM application covered in Part II manages patient risks by identifying, evaluating, and managing risks associated with process parameters (PP) on the product’s critical quality attributes (CQAs). The final paper, Part III, describes an approach for accepting or further mitigating the risks evaluated by the QRM exercise...

Citation:
Witcher MF. Quality risk management (QRM): part I – identifying, evaluating, and mitigating threat risks to biopharmaceutical enterprises. BioProcess J, 2016; 15(3): 21–9. https://doi.org/10.12665/J153.Witcher.

Posted online November 15, 2016.

 
Monoclonal Antibody Generation and Characterization for Vip3Aa20 Protein Quantification in Transgenic Corn Plants

by Daily Hernández, Hasel Aragón, Marcos González, Gabriela González, Amarilis González, Andrés Tamayo, Ivis Morán, Pilar Téllez, Eduardo Sánchez, Miguel Castillo, Williams Ferro, Camilo Ayra, and Rodolfo Valdés
Volume 15, Issue 3 (Fall 2016)

Numerous standardized techniques for detection and quantification of proteins are based on polyclonal antibody (pAb) use. However, because pAbs are a heterogeneous mixture of antibodies, there is the possibility of non-specific interactions or cross-reactions with non-related proteins, which is a disadvantage in the detection and quantification of target proteins. Therefore, the main objective of this study was to generate and characterize monoclonal antibodies (mAbs) for quantifying the Vip3Aa20 protein of Bacillus thuringiensis (Bt) expressed in event MIR162 transgenic corn plants.

The Vip3Aa20 bioinsecticidal protein of Bt shares 99.7% of homology with the Vip3Aa1 protein, which has already been successfully isolated by affinity chromatography on metal chelate. For this reason, Vip3Aa1 was chosen as an antigen for obtaining mouse mAbs directed against these Bt δ-endotoxins. The mAbs were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)/Western blot, high-pressure liquid chromatography gel filtration (HPLC-GF), enzyme-linked immunosorbent assay (ELISA), and association constant. In general, results showed that the use of the highly purified mAbs not only allowed the specific and accurate quantification of Vip3Aa20 in extracts of macerated leaves of event MIR162 transgenic corn plants, but also statistically discriminated (p=0.000) between homozygous (range: 180–360 ng/mL, mean ± standard deviation [SD]: 241.7 ± 43.2 ng/mL) and hemizygous (range: 0–140 ng/mL, mean ± SD: 51.7 ± 40.8 ng/mL) transgenic corn plants grown under the same experimental conditions...

Citation:
Hernández D, Aragón H, González M, González G, González A, Tamayo A, Morán I, Téllez P, Sánchez E, Castillo M, Ferro W, Ayra C, Valdés R. Monoclonal antibody generation and characterization for Vip3Aa20 protein quantification in transgenic corn plants. BioProcess J, 2016; 15(3): 30–43. https://doi.org/10.12665/J153.Valdes.

Posted online November 15, 2016.

 
Eco-Friendly Red Pigment Production by Sporosarcina aquimarina

by Vigi Chaudhary, Aditi Goyal, Jignesh Chaudhary, and A.N. Pathak
Volume 15, Issue 3 (Fall 2016)

Acetyl-4, 4′-diapolycopene-4, 4′-dioate, a C30 carotenoid and secondary metabolite, was produced by the Sporosarcina aquimarina bacteria using a 5.0 L fermentation vessel with a 3.0 L working volume. In the presence of tryptone, the biosynthesis of acetyl-4, 4′-diapolycopene-4, 4′-dioate production using a batch fermentation process was further improved. Production parameters like carbon source, pH, and temperature were studied, and maximum product was achieved, up to 1.2 g/L, where the secondary metabolite yield was 0.07 g/L and productivity, 0.00833 g/L/h. The organic constitution and significant red color intensity of the acetyl-4, 4′-diapolycopene-4, 4′-dioate molecule can be used in the textile industry as a dye, and a coloring additive in processed foods and pharmaceuticals...

Citation:
Chaudhary V, Goyal A, Chaudhary J, Pathak AN. Eco-friendly red pigment production by Sporosarcina aquimarina. BioProcess J, 2016; 15(3): 44–51. https://doi.org/10.12665/J153.Pathak.

Posted online November 15, 2016.

 
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